The Audhya laboratory consists of approximately 1000 square feet of designated laboratory space on the ninth floor of WIMR Tower II with equipment for biochemistry, live cell confocal microscopy, image analysis, molecular biology, and mammalian tissue culture cell maintenance. This space includes 10 laboratory benches, furnished office space (see below), two 200 sq. ft. confocal microscopy suites, two 100 sq. ft. tissue culture rooms (one dedicated specifically to human induced pluripotent stem cell growth and maintenance), and a large 100 sq. ft. cold room. As an affiliate of the Waisman Center at UW-Madison, the PI also has access to a large rodent housing facility, which includes space for a variety of procedures including tissue harvesting for ex vivo cell culture. This space includes a shared 100 sq. ft. tissue culture room and a large behavioral suite dedicated to the study of rats. The Audhya laboratory holds multiple computers, including a state of the art Synology RackStation RS3618XS – NAS server with a hard drive capacity of 384 terabytes, an additional Macintosh Pro Server with a hard drive capacity of 10 terabytes, a Macbook connected to a 24” cinema display, several Macbook laptop computers, including a laptop dedicated to rodent gait analysis, an Apple iMac, three IBM PC computers (connected to two FPLC systems and a confocal microscopy system), an advanced PC workstation for running IMOD (tomographic 3D rendering), two additional advanced PC workstations for running Imaris and MetaXpress (microscopy image analysis software programs), and two laser printers. All advanced workstations are housed within a 200 square foot compute area that is directly adjacent to the main laboratory. All computers share a common network. In addition, the Audhya lab also continues to utilize 300 square feet of designated laboratory space on the fifth floor of the Biochemical Sciences Building with equipment for biochemistry and molecular biology. This space includes a total of 3 laboratory benches and a large hood used for lipid biochemistry.
Spinning disk confocal microscope
- Ideal for live cell imaging
- Perfect focus system
- Stage-top incubator for imaging long-term
- High resolution CCD camera
- 4 imaging laser lines (405, 488 nm, 561 nm, 647 nm)
- Piezo equipped stage for fast, accurate z-positioning
- coming soon: TIRF and photostimulation/photobleaching capabilities
Swept-field confocal microscope
- Ideal for live cell imaging
- Perfect focus system
- Stage-top incubator for imaging long-term
- High resolution CCD camera
- 3 imaging laser lines (488 nm, 561 nm, 638 nm)
- 405 nm laser for photoactivation and photobleaching studies
AKTA purifier (UPC10)
- FPLC protein purification system
- Automated fraction collector
- Ion exchange chromatography
- Size exclusion chromatography
- In-line MALS detector for absolute molecular mass determination
AKTA purifier (Pure L1)
- FPLC protein purification system
- Automated fraction collector
- Ion exchange chromatography
- Size exclusion chromatography
Wyatt miniDAWN TREOS
- Multi-angle light scattering device
- Coupled to AKTA purifier
- Determination of absolute molecular mass
MicroCal ITC
- Small volume titration calorimeter
- Characterization of molecular interactions
- True affinity data (in solution) via heat measurement
PTI QM40 Spectrofluorometer (Shared)
- Automated monochromators
- Extended 185 – 900 nm wavelength range
- Stopped-flow accessory for rapid mixing experiments
Beckman DU 730 spectrophotometer
- Rapid measurement of protein and nucleic acid concentration
- Microcuvette assembly for small volume measurements
- Measurement of dye incorporation
Vesicle Prep Pro
- Electroformation of giant unilamellar vesicles
- Temperature controlled
- Amenable to use with variable lipid compositions
Gradient Master (shared)
- Rapidly generates gradients for sedimentation assays
- Compatible with a wide range of gradient solutions
Microinjection system
- NARISHIGE microinjector on a Zeiss Axiovert platform
- Injection of nucleic acids into C. elegans
- Accompanying needle puller
Tissue culture suite
- Large biosafety cabinet
- Multiple CO2 incubators
- Microscope for viewing cells with multiple objectives
- Incubator for baculovirus production
Walk-in cold room (Shared)
- Ideal for initial steps of protein purification
- High capacity storage available
Centrifugation equipment
- Avanti J-26 XP (large capacity)
- Beckman TL100 (tabletop ultracentrifuge)
- Sorvall RC-5B (floor model centrifuge)
- Thermo IEC31R (clinical centrifuge)
